Figure 2From: Co-overexpression of two Heat Shock Factors results in enhanced seed longevity and in synergistic effects on seedling tolerance to severe dehydration and oxidative stressWestern analyses of HSP accumulation in seeds from double homozygous DS10:A9/A4a lines and sibling DS10:A9 lines. The depicted line pairs represent the two A9 genetic backgrounds: A91 and A92. (A) 1D-western analyses; the antibodies used for immunodetection are indicated on the right. These include: HA (anti-hemaglutinin) and the anti-HSP antibodies specific for sHSP CI, sHSP CII and HSP101. (B) 2D-western analyses of sHSP CII accumulation. The asterisks and a thin circle mark polypeptides with enhanced accumulation in the DS10:A91/A4a seeds. The pH range for isoelectric focusing is indicated (bottom). Molecular mass markers (in kDa) are indicated on the left.Back to article page