Skip to main content
Figure 6 | BMC Plant Biology

Figure 6

From: Involvement of co-repressor LUH and the adapter proteins SLK1 and SLK2 in the regulation of abiotic stress response genes in Arabidopsis

Figure 6

Histone H3 and H2B interacts with LUH in Yeast Two Hybrid and in planta . (A) LUH was expressed from bait vector as BD fusion and H3 or H2B from prey vector as AD fusion. Results of yeast two hybrid assay indicating the activation of α- Galactosidase reporter expression. Blue color indicates positive interaction. (B) Arabidopsis protoplasts were transfected with 15 μg of each plasmid containing LUH as N-LUC fusion and H3 or H2B as C-LUC fusion. CaMV 35S::Renilla LUC reporter was used as an internal control for transfection and LUC activity is expressed relative to the R-LUC activity. Error bars are SE (n = 3). Asterisks indicate values that are significantly different from the vector control (*P <0.05, Student’s t test).

Back to article page