Figure 6From: Involvement of co-repressor LUH and the adapter proteins SLK1 and SLK2 in the regulation of abiotic stress response genes in ArabidopsisHistone H3 and H2B interacts with LUH in Yeast Two Hybrid and in planta . (A) LUH was expressed from bait vector as BD fusion and H3 or H2B from prey vector as AD fusion. Results of yeast two hybrid assay indicating the activation of α- Galactosidase reporter expression. Blue color indicates positive interaction. (B) Arabidopsis protoplasts were transfected with 15 μg of each plasmid containing LUH as N-LUC fusion and H3 or H2B as C-LUC fusion. CaMV 35S::Renilla LUC reporter was used as an internal control for transfection and LUC activity is expressed relative to the R-LUC activity. Error bars are SE (n = 3). Asterisks indicate values that are significantly different from the vector control (*P <0.05, Student’s t test).Back to article page