Table 2 The primer sequences, annealing temperatures (Ta) and number of cycles for semi-quantitative PCR reactions
Gene | Primer’s name | The sequence of the primer | Ta [°C] | Cycles | Organism and GenBank number |
|---|---|---|---|---|---|
Actin | ACTforward | 5’- CCGGTGTTATGGTTGGAAT-3’ | 61 | 23 | Linum usitatissimum actin (Act1), [GenBank: AY857865.1] |
ACTreverse | 5’- TGTAGAAAGTGTGATGCCAAA-3’ | 61 | 23 | ||
CAD | CADforward | 5’- GGAGCATGAAGGAAACAGAG-3’ | 62 | 27 | Linum ustatissimum L. cinnamyl alcohol dehydrogenase (CAD), [GenBank: DQ487210.1] |
CADreverse | 5’- CTACCTACGGAGGCTACT-3’ | 57 | 27 | ||
SAD | SADforward | 5’- CAACATCAACCACGAACCTA-3’ | 61.5 | 27 | Linum usitatissimum scaffold726_21, [GenBank AFSQ01019765.1] on the basis of Populus tremuloides sinapyl alcohol dehydrogenase, [GenBank: AF273256] |
SADreverse | 5’- GCTTGTCTAGCCCATAGAAC-3’ | 58.5 | 27 |