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Table 2 The primer sequences, annealing temperatures (Ta) and number of cycles for semi-quantitative PCR reactions

From: Manipulating cinnamyl alcohol dehydrogenase (CAD) expression in flax affects fibre composition and properties

Gene Primer’s name The sequence of the primer Ta [°C] Cycles Organism and GenBank number
Actin ACTforward 5’- CCGGTGTTATGGTTGGAAT-3’ 61 23 Linum usitatissimum actin (Act1), [GenBank: AY857865.1]
ACTreverse 5’- TGTAGAAAGTGTGATGCCAAA-3’ 61 23
CAD CADforward 5’- GGAGCATGAAGGAAACAGAG-3’ 62 27 Linum ustatissimum L. cinnamyl alcohol dehydrogenase (CAD), [GenBank: DQ487210.1]
CADreverse 5’- CTACCTACGGAGGCTACT-3’ 57 27
SAD SADforward 5’- CAACATCAACCACGAACCTA-3’ 61.5 27 Linum usitatissimum scaffold726_21, [GenBank AFSQ01019765.1] on the basis of Populus tremuloides sinapyl alcohol dehydrogenase, [GenBank: AF273256]
SADreverse 5’- GCTTGTCTAGCCCATAGAAC-3’ 58.5 27
  1. AKT actin; CAD cinnamyl alcohol dehydrogenase; SAD sinapyl alcohol dehydrogenase.