Figure 2
Multiple alignment of Arabidopsis and predicted Pinophyta CLEL protein amino acid sequences. A schematic diagram of a generic CLEL protein representing the main features of CLEL proteins is shown above the alignment. The amino (H2N) terminus of the schematic protein is followed by the signal peptide (SP), the first non-conserved sequence (NCS1), the CLEL domain (CLD) and the second non-conserved sequence (NCS2) found at the COOH terminus of some CLEL proteins. Presumed cleavage sites of the SP and the mature CLEL peptide sequence are indicated by large arrowheads. The multiple alignment depicts the individual SPs of each putative full-length protein sequence with grey highlighting. The SignalP 4.1-predicted cleavage sites are indicated by the small arrowheads. The CLEL motif, including the two conserved asp-tyr amino acids at the amino termini of the predicted CLEL peptides, is indicated by white lettering. The predicted CLEL peptides are indicated by black highlighting except for the asp-tyr sequence, which is indicated by brick red highlighting.