Figure 7

Comparison of the levels of proline, MDA and chlorophyll between WT and EsDREB2B -transformed tobacco after osmotic, salt, cold and heat stresses. For osmotic and salt stresses, the two-week-old seedlings grown in MS medium under normal conditions (28°C) transplanted to MS medium supplemented with 200 mM NaCl and 200 mM mannitol and stressed for 20 d; for cold and heat stresses, the two-week-old seedlings grown in MS medium and 28°C were treated for 20 d at 4°C and 7 d at 42°C, respectively. Seedlings were kept growing in MS culture and 28°C is the non-stress control (NS). The lethal seedlings were not collected for physiology index measuring. A MDA content, B proline content, C chlorophyll content. Values are mean ± SE from three independent experiments (24 plants of each line were grown for each experiment). a–c: there are no significant differences (p ≥ 0.05) between the lines with the same characters, and significant differences (p < 0.05) between lines with different characters. Comparison was carried out within stress.