Figure 5From: EsDREB2B, a novel truncated DREB2-type transcription factor in the desert legume Eremosparton songoricum, enhances tolerance to multiple abiotic stresses in yeast and transgenic tobaccoGrowth of S. cerevisiae yeast cells transformed with the empty vector (1) PYES2 and with the (2) PYES2-EsDREB2B under different stress conditions. To test the osmotic and salt tolerances, the same quantity of yeast culture sample was re-suspended in 30% PEG6000 and 5 M NaCl, respectively, at 30°C for 36 h. To test the cold and heat tolerances, the yeast cells were re-suspended in SC-ura medium and placed either in a refrigerator at -20°C alcohol bath or in a water bath at 53°C, respectively, for 24 h. To test oxidative tolerance, yeast cells were incubated in 20 mM H2O2 at 30°C for 8 h. For non-stress, control (NS), an equivalent number of yeast cells was re-suspended in 200 μl of sterile water and incubated at 30°C for 36 h. Serial dilutions of 1:10 transformed yeast cells were grew on SC-Ura medium for 3 d. A non-stress (NS), B osmotic stress, C salt stress, D cold stress, E heat stress and F oxidative stress.Back to article page