Figure 4From: EsDREB2B, a novel truncated DREB2-type transcription factor in the desert legume Eremosparton songoricum, enhances tolerance to multiple abiotic stresses in yeast and transgenic tobaccoSubcellular localization and transactivation analysis of EsDREB2B protein. A GFP and EsDREB2B: GFP fusion protein were transiently expressed under the control of the CaMV 35S promoter in onion epidermal cells and observed with a laser scanning confocal microscope. The images were presented fluorescence, bright field and merge of bright field and fluorescence. B The transcriptional activation ability of EsDREB2B was examined using the yeast one-hybrid assay. The growth of yeast cells on SD/-His-Trp medium and C β-galactosidase activity was analyzed. PBridge-BD vector served as the negative control and pBridge-BD-GAL4 served as the positive control.Back to article page