The C-terminal propeptide of a plant defensin confers cytoprotective and subcellular targeting functions

BMC Plant Biology

Table 3 NaD1 levels and phenotype of segregating pHEX22 T₁plants expressing SP-NaD1ΔCTPP and homozygous Line D1 plants expressing SP-NaD1-CTPP

Line	Zygosity*	Plant no.	NaD1 ppm	Leaf distortion	Fertile	Bolls	Seed	Germination %
78.131.1	Hom	10	5.0	Severe	Yes	1	11	55
		23	5.9	Severe	No	0
		34	7.9	Severe	No	0
		42	8.3	Severe	Yes	1	7	57
		Ave.	6.8
78.131.1	Hem	48	2.7	Moderate	Yes	2	20	70
		12	3.0	Moderate	Yes	1	17	100
		44	2.4	Moderate	Yes	2	38	80
		47	2.2	Moderate	Yes	2	16	80
		Ave.	2.6
83.102.1	Hom	7	1.5	Mild	Yes	2	25	90
		20	2.0	Mild	Yes	2	40	80
		21	0.6	Mild	Yes	4	39	100
		27	2.6	Mild	Yes	3	33	100
		Ave.	1.7
83.102.1	Hem	15	0.8	Slight	Yes	4	31	90
		16	0.7	Slight	Yes	4	43	80
		25	0.9	Slight	Yes	2	49	100
		29	1.0	Slight	Yes	3	32	80
		Ave	0.9
83.68.1	MC	1	0.6	None	Yes
		2	0.7	None	Yes
		3	1.1	None	Yes
		4	1.0	None	Yes
		5	1.0	None	Yes
		6	2.1	None	Yes
		Ave	1.1
83.23.2	MC	2	0.2	None	Yes
		3	0	None	Yes
		4	3.0	None	Yes
		5	0	None	Yes
		6	0.1	None	Yes
		7	0	None	Yes
		Ave	0.6
D1	Hom	1	2.7	None	Yes
		2	3.6	None	Yes
		3	4.1	None	Yes
		Ave	3.5

*Hom = homozygous, Hem = hemizygous, MC = multicopy lines, zygosity not determined. NaD1 levels were determined 6 weeks after sowing. ppm = ng NaD1/mg wet weight tissue.

ISSN: 1471-2229