Figure 1

Production of NaD1 in leaves of transgenic cotton transformed with pHEX22 or pHEX3. (A) Diagram of the defensin precursors encoded by pHEX3 and pHEX22. Both precursors have ER signal peptides that direct the protein into the secretory pathway. pHEX22 encodes the mature NaD1 defensin of 5.3 kDa and pHEX3 encodes NaD1 with a C-terminal propeptide. This propeptide is removed from the ~9 kDa intermediate during transit to or storage in the vacuole where the mature 5.3 kDa defensin accumulates. Processing sites are indicated by black arrows. (B) Immunoblot of leaf tissue using an anti-NaD1 antibody. Lane 1: 50 ng purified NaD1 from N. alata flowers, lane 2: 150 ng purified NaD1 from N. alata flowers, lane 3: line D1 (homozygous T₃), lane 4: line 78.131.1 (T₁), lane 5: line 83.68.1 (T₁), lane 6: 83.68.1 (T₁), lane 7: non-transgenic Coker 315. Size markers are SeeBlue Plus2 (Invitrogen).