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Table 2 Correlation analyses between the relative expression (2-ΔΔCt) of the biosynthetic and regulatory PA genes and the PA content (mg PAs g-1of dry weight); Pearson test

From: Lotus tenuis x L. corniculatus interspecific hybridization as a means to breed bloat-safe pastures and gain insight into the genetic control of proanthocyanidin biosynthesis in legumes

Leaves and stems of parental and F1 hybrids
Gene Soluble PAs Insoluble PAs Total PAs TT2 bHLH
Correlation coefficient p-value Correlation coefficient p-value Correlation coefficient p-value Correlation coefficient p-value Correlation coefficient p-value
TT2 0.83 0.0008 0.97 <0.0001 0.91 <0.0001     
bHLH -0.36 0.2500 -0.11 0.7500 -0.25 0.4200     
PAL -0.31 0.3300 -0.50 0.1000 -0.40 0.2000 -0.49 0.0010 -0.002 0.90
CHS 0.78 0.0029 0.90 <0.0001 0.85 0.0005 0.79 <0.0001 0.07 0.68
DFR 0.99 <0.0001 0.92 <0.0001 0.98 <0.0001 0.82 <0.0001 -0.27 0.09
ANS 0.70 0.0100 0.76 0.0045 0.74 0.0100 0.70 <0.0001 -0.30 0.05
ANR 0.82 0.0010 0.92 <0.0001 0.88 0.0002 0.89 <0.0001 -0.18 0.26
LAR1 0.97 <0.0001 0.98 <0.0001 0.99 <0.0001 0.91 <0.0001 -0.23 0.15
LAR2 0.99 <0.0001 0.89 <0.0001 0.97 <0.0001 0.78 <0.0001 -0.32 0.04
Leaves of parental and selected F2 plants
Gene Soluble PAs Insoluble PAs Total PAs TT2 bHLH
Correlation coefficient p-value Correlation coefficient p-value Correlation coefficient p-value Correlation coefficient p-value Correlation coefficient p-value
TT2 0.85 <0.0001 0.86 <0.0001 0.87 <0.0001     
bHLH -0.31 0.1400 -0.15 0.5000 -0.21 0.3200     
PAL -0.29 0.1800 -0.19 0.3800 -0.23 0.2800 -0.01 0.9500 0.29 0.1700
CHS 0.41 0.0500 0.41 0.0400 0.42 0.0400 0.29 0.1700 0.08 0.7100
DFR 0.87 <0.0001 0.81 <0.0001 0.85 <0.0001 0.71 0.0001 -0.32 0.1300
ANS 0.79 <0.0001 0.80 <0.0001 0.81 <0.0001 0.88 <0.0001 -0.28 0.1900
ANR 0.71 0.0001 0.75 <0.0001 0.75 <0.0001 0.88 <0.0001 -0.18 0.4100
LAR1 0.89 <0.0001 0.91 <0.0001 0.92 <0.0001 0.89 <0.0001 -0.12 0.5800
LAR2 0.83 <0.0001 0.84 <0.0001 0.85 <0.0001 0.66 0.0004 -0.47 0.0200