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Table 2 Correlation analyses between the relative expression (2-ΔΔCt) of the biosynthetic and regulatory PA genes and the PA content (mg PAs g-1of dry weight); Pearson test

From: Lotus tenuis x L. corniculatus interspecific hybridization as a means to breed bloat-safe pastures and gain insight into the genetic control of proanthocyanidin biosynthesis in legumes

Leaves and stems of parental and F1 hybrids

Gene

Soluble PAs

Insoluble PAs

Total PAs

TT2

bHLH

Correlation coefficient

p-value

Correlation coefficient

p-value

Correlation coefficient

p-value

Correlation coefficient

p-value

Correlation coefficient

p-value

TT2

0.83

0.0008

0.97

<0.0001

0.91

<0.0001

    

bHLH

-0.36

0.2500

-0.11

0.7500

-0.25

0.4200

    

PAL

-0.31

0.3300

-0.50

0.1000

-0.40

0.2000

-0.49

0.0010

-0.002

0.90

CHS

0.78

0.0029

0.90

<0.0001

0.85

0.0005

0.79

<0.0001

0.07

0.68

DFR

0.99

<0.0001

0.92

<0.0001

0.98

<0.0001

0.82

<0.0001

-0.27

0.09

ANS

0.70

0.0100

0.76

0.0045

0.74

0.0100

0.70

<0.0001

-0.30

0.05

ANR

0.82

0.0010

0.92

<0.0001

0.88

0.0002

0.89

<0.0001

-0.18

0.26

LAR1

0.97

<0.0001

0.98

<0.0001

0.99

<0.0001

0.91

<0.0001

-0.23

0.15

LAR2

0.99

<0.0001

0.89

<0.0001

0.97

<0.0001

0.78

<0.0001

-0.32

0.04

Leaves of parental and selected F2 plants

Gene

Soluble PAs

Insoluble PAs

Total PAs

TT2

bHLH

Correlation coefficient

p-value

Correlation coefficient

p-value

Correlation coefficient

p-value

Correlation coefficient

p-value

Correlation coefficient

p-value

TT2

0.85

<0.0001

0.86

<0.0001

0.87

<0.0001

    

bHLH

-0.31

0.1400

-0.15

0.5000

-0.21

0.3200

    

PAL

-0.29

0.1800

-0.19

0.3800

-0.23

0.2800

-0.01

0.9500

0.29

0.1700

CHS

0.41

0.0500

0.41

0.0400

0.42

0.0400

0.29

0.1700

0.08

0.7100

DFR

0.87

<0.0001

0.81

<0.0001

0.85

<0.0001

0.71

0.0001

-0.32

0.1300

ANS

0.79

<0.0001

0.80

<0.0001

0.81

<0.0001

0.88

<0.0001

-0.28

0.1900

ANR

0.71

0.0001

0.75

<0.0001

0.75

<0.0001

0.88

<0.0001

-0.18

0.4100

LAR1

0.89

<0.0001

0.91

<0.0001

0.92

<0.0001

0.89

<0.0001

-0.12

0.5800

LAR2

0.83

<0.0001

0.84

<0.0001

0.85

<0.0001

0.66

0.0004

-0.47

0.0200