ABA responsiveness of LeMYC2 transgenic plants. A, Real time PCR analysis of LeMYC2 expression in 6-day-old tomato seedlings treated with ABA for 48 h. No hormone treatment was used as control (Mock), and different concentrations of ABA are indicated. B and C, Photographs showing difference in the germination of 3-day-old control (VEC) and various LeMYC2 transgenic lines, grown in absence and presence of 5 μM ABA respectively. In both Figures, from left to right, seedlings of VEC (vector control), LeMYC2OE1 and LeMYC2Ri1 lines are shown. The seeds are considered to be germinated while the radicle tip had fully expanded the seed coat. The experiments were repeated thrice and similar results were obtained. The data shown is the representative of one of those experiments. D, Quantification of percentage of seeds germinated over a period of time as shown in B. The error bars indicate SD (n = 3). E to G, Real time PCR analysis of ABA marker genes, AREB(D), PP2C(E) and TAS14(F) from 6-day-old controls (UN and VEC) and various LeMYC2 transgenics in WL (60 μmol m-2 s-1) and transferred to MS or MS with 5 μM ABA for 6 h in WL. The error bars indicate SD (n = 3), normalized with LeACTIN. Accession numbers of genes are AREB (AY530758.1), TAS14 (X51904.1) and PP2C (AI772677.1).