Skip to main content

Table 1 Sequences of oligonucleotide primers used in this study

From: GIGANTUS1 (GTS1), a member of Transducin/WD40 protein superfamily, controls seed germination, growth and biomass accumulation through ribosome-biogenesis protein interactions in Arabidopsis thaliana

Name

Primer sequence

Description

GTS1-F1

5’GAGGAGCTGCAGGGTTATTT3’

For RT-PCR

GTS1-R1

5’CAAGACGGGTTAATCTGGGTAG3’

For RT-PCR

TDNA-LB

5’CCGTCTCACTGGTGAAAAGAA3’

For TDNA insertion

GTS1-F2

5’CTGAAACGGCAAATGGAAGAAG3’

For complementation test

GTS1-R2

5’CTATGTTGCTGGAAGTCGGAT3’

For complementation test

Act2-F

5’GCGGATCCATGGCTGAGGCTGATGATATTCAACC3’

For RT-PCR

Act2-R

5’CGTCTAGACCATGGAACATTTTCTGTGAACGATTCC3’

For RT-PCR