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Figure 2 | BMC Plant Biology

Figure 2

From: GIGANTUS1 (GTS1), a member of Transducin/WD40 protein superfamily, controls seed germination, growth and biomass accumulation through ribosome-biogenesis protein interactions in Arabidopsis thaliana

Figure 2

Physical map of GTS1 knockout gene and phenotypic characterization of gts1 mutant. (A) The GTS1 gene with the positions of exons (numbered black rectangles) and introns (thick lines) are represented. The 5’ and 3’ untranslated regions are depicted in white rectangles. The location of the gts1 T-DNA insertion is shown using an inverted black triangle. The names and locations of primers used for RT-PCR experiments are also indicated. Bar = 0. 5 kb. (B) The T-DNA insertion causes a knockout expression of the gene. The quality of the RNA and the loading control was assayed by monitoring ACTIN gene expression. (C-F) GTS1 negatively controls seed germination. gts1 mutant germinated faster at 1 and 3 days after incubation in water (D, F) than the wild type (C, E). (G-H) GTS1 controls biomass accumulation and growth development in Arabidopsis. (H), Growth rate of gts1 is faster than that of WT (G) at 15 DAG. gts1 shows larger leaf area (biomass) (H) than WT (G). DAG = Days after germination.

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