Effects of Bacillus megaterium inoculation on gene expression. Analysis of Sl-PR1b, Sl-ACO4, Sl-ACS7, Sl-NCED and Sl-LE16 gene expression by real time quantitative RT-PCR in leaves of wild-type cv Ailsa Craig tomato plants and mutant flacca inoculated with Bacillus megaterium. Treatments are designed as non-inoculated plants (white bars) or inoculated wild-type or flacca plants (black bars). Data represent the mean values ± SE of four independent biological replicates. Means followed by asterisk are significantly different with respect to corresponding non-inoculated control (P < 0.05) as determined by t-student test. P values for two-way ANOVA are reported in Table 2.