Exploring mechanisms linked to differentiation and function of dimorphic chloroplasts in the single cell C4 species Bienertia sinuspersici

Table 2 SEM backscattering quantification of Bienertia DAB staining, using Image J analysis to quantify the Integrated Density of the two chloroplast types

Stain	Total average CCC/PCC density ratio
Control stain	1.658 +/− 0.128*
H₂O₂ stain	1.265 +/− 0.097*
Peroxidase control stain	1.196 +/− 0.058*
Peroxidase stain	1.965 +/− 0.239*

The density ratio was calculated by dividing the average integrated density of 5–7 central compartment chloroplasts (CCC) by the average integrated density of an equal number of peripheral chloroplast (PCC) for each cell. The total average CCC/PCC density ratio was obtained by averaging the density ratio from two biological replicates (3 cells each) and two technical replicates. The standard error is shown for all 12 measurements. ^*Difference between the control and stain are statistically significant at p < 0.05, using a independent T-test.

ISSN: 1471-2229