Exploring mechanisms linked to differentiation and function of dimorphic chloroplasts in the single cell C4 species Bienertia sinuspersici

Table 1 The average CCC/PCC ratio of GFP fluorescence from the dimorphic chloroplast of transformed Bienertia protoplasts, using the constructs RbcS-FL spGFP and RbcS-FL roGFP2, where the roGFP2 protein is redox sensitive while the spGFP protein is redox insensitive

Construct	# of quantified cells	Average CCC/PCC ratio of fluorescence
RbcS-FL spGFP	31	1.028 +/− 0.024*
RbcS-FL roGFP2	28	1.439 +/− 0.005*

Lambda mode on the Zeiss 510 confocal microscope was used to measure the intensity of GFP fluorescence. Fluorescence intensity values were obtained using an excitation wavelength of 488 nm, and detection of emission at a wavelength of 513 nm. (Additional file 6: Figure S5 shows a picture of the analysis). The ratio of GFP fluorescence intensity between the two chloroplast types was calculated by dividing the intensity of one central compartment chloroplast by the intensity of one peripheral compartment chloroplast. The ratio was averaged across the number of quantified cells. The results presented are from 7 biological replicates. The standard error from all measurments is shown. *Difference between the two constructs are statistically significant at p < 0.05, using a independent T-test.

ISSN: 1471-2229