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Figure 5 | BMC Plant Biology

Figure 5

From: Exploring mechanisms linked to differentiation and function of dimorphic chloroplasts in the single cell C4 species Bienertia sinuspersici

Figure 5

Estimation of PSII content in Bienertia chlorenchyma cells, using confocal microscopy. Cells were excited with 633 nm of light, and the brighter areas in images A, B, and C have greater fluorescence intensities, corresponding to more PSII content. A) Lambda mode image showing fluorescence from the two chloroplast types of Bienertia. Cursor 1 was placed on one peripheral chloroplast, while cursor two was placed on one central compartment chloroplast. B) Lambda mode image showing fluorescence from the two chloroplast types of Zea mays. Cursor 1 was placed on the mesophyll chloroplasts, while cursor two was placed on the bundle sheath. C) Heat map of a z-stack (serial images) series of a Bienertia protoplast at 1 μm slices, intensity represents the emission at 684 nm using 633 nm excitation. D) Quantification of the emission spectra of the two types of Bienertia chloroplasts; squares are peripheral compartment chloroplasts, and diamonds are central compartment chloroplasts. Error bars represent the standard deviation for 8 different protoplasts.

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