Figure 3
Immunolocalization of C 4 proteins in single cell type Bienertia and Kranz type Suaeda taxifolia . Confocal microscopy detection of the Alexa fluor 546 tagged secondary antibody, reacting to the primary antibodies indicated, showing the location of phosphoenolpyruvate carboxylase (PEPC, A-D), rubisco large subunit (rbcL, E-H), and rubisco large subunit mRNA binding protein (RLSB, I-L) in Bienertia (A, B, E, F, I, and J) and Suaeda taxifolia (C, D, G, H, K, and L). Images A, C, E, G, I, and K are Alexa Fluro 546 detection. Images B, D, F, H, J, and L are bright field view of sections. CCC = central compartment chloroplast, PCC = peripheral compartment chloroplast, M = Mesophyll Cell, BS = Bundle Sheath Cell. Scale bar = 50 μm.