Figure 3

Analysis of ASR1-bound genomic regions. A) Distribution of ASR1-binding regions along the genome. The percentage of peaks located 5’ (up to 3 kb upstream of the start point of transcription), within the gene bodies and 3’ (down to 1 kb downstream of the end of transcription). B) Cellular functions of the enriched genes present in peaks. Classification was based on reported gene functions. Small categories comprising less than 2% of the genes were grouped and referred to as “other categories” instead of “miscellaneous”. C-D-E) Average distribution of leaf ASR1 protein upon stress along distinct tomato gene regions. For each region displayed in A, we considered only those genes that gave reads mainly in each visualized category (66 genes for “promoter” (C), 72 genes for “exons” (D) and 21 genes for “downstream regions” (E)). Each gene region was subdivided into 100 consecutive segments. The X-axis represents the segment number. The number of reads in each segment (for each gene) was relativized to the number of reads of the gene that showed the highest peak. Normalized values from all the genes for each segment were then averaged (Y-axis) to display any bias in the location of ASR1 binding within each region.