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Figure 2 | BMC Plant Biology

Figure 2

From: Genome-wide data (ChIP-seq) enabled identification of cell wall-related and aquaporin genes as targets of tomato ASR1, a drought stress-responsive transcription factor

Figure 2

Visual verification of the peaks and individual validation. A) A histogram indicating the number of reads of 150-bp window DNA sequences along five selected genomic regions (3–830, 9–950, 10–800, 10–810 and 10–820) where statistically significant peaks were observed. The corresponding gene organization in exons (boxes) and introns is also shown. The +1 arrow indicates the sense of transcription. ST-3 yielded no peaks. B) qPCR for the same five selected sequences present in peaks from ChIP-seq. For each locus, the % INPUT is shown. The ST-3 amplicon was used as a negative control. Amplicon names are abbreviations for gene codes (see Table 2, which also shows gene function). Primers are listed in Additional file 1: Table S1. *p < 0.05, **p < 0.01, ***p < 0.005 (a one-tailed Student’s t-student compared against ST-3).

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