Figure 5
Constitutive cytokinin responses of etiolated seedlings expressing phosphomimic ARR1. (A) Four-day-old 35S:ARR1 overexpressing (WT-ox) and 35S:ARR1D94E overexpressing (PM-ox) etiolated seedlings are shown. Scale bar = 2 mm. (B) Hypocotyl length of four-day-old seedlings was measured from photographs using ImageJ and is presented as mean ± SD (n ≥ 20). Unless marked differently, lines were compared to Col-0 using a one-way ANOVA with Tukey’s multiple comparison test. **, P < 0.01 and ****, P < 0.0001. (C) Four-week-old etiolated seedlings. Scale bar = 2 mm. (D) The length of the cotyledonary petioles was measured from photographs using ImageJ and presented as mean ± SD (n ≥ 12). **, P < 0.01, ***, P < 0.001 and ****, P < 0.0001 for one-way ANOVA with Tukey’s multiple comparison test. ns, not statistically significant (P > 0.05).