Figure 1

Analyses of ARR1 expression levels in phosphomimic 35S:ARR1^D94E( PM- ox ) and 35S:ARR1 ( WT- ox ) lines and the characteristic dwarf phenotype of 35S:ARR1^D94E plants. (A) The ARR1 levels in the Col-0 wild type, the arr1-1 mutant and the overexpression lines generated in the arr1-1 background were determined using anti-ARR1 antibody. Immunoblotting analysis with the anti-α-tubulin (TUA) antibodies and the image of the region of the Ponceau S-stained membrane surrounding the large RuBisCO subunit (LSU) are shown as controls. L, low and H, high expression levels. (B) Quantification of ARR1 expression levels. Chemiluminescent ARR1 signals were normalized to TUA and results are expressed as fold increase compared to Col-0, which was assigned a value of 1. Data are presented as mean ± SEM of two independent experiments. (C) Rosettes of representative two-week-old plants grown on MS/2 media.