Transcript accumulation in barley inoculated with different Magnaporthe isolates and pre-treated with the fungicide Beam. Using RT-qPCR the relative transcript abundance of CYP96B22 (A) and PR1b (B) in barley epidermal strips at 6, 12, 24 and 48 h after inoculation with a Magnaporthe host (TH6772) or nonhost isolate (CD180) or a mutant (∆pmk1) was determined. One group of plants was treated with the fungicide Beam prior to inoculation and the other with water (control treatment). Transcript abundance was calculated for each sample relative to the reference gene EF1α and is shown as the mean and standard error of three independent biological replicates. For normalization of differences in the absolute values among the biological replicates, the expression values were calculated in each experiment relative to the mock-inoculated sample (control treatment) at 6 h p.i. which was set to 1.0. Statistical analysis of CYP96B22 expression was done only for the 6 h p.i. time point by comparing values to the mock-inoculated sample of the control treatment at 6 h p.i. using a paired t-test. Samples are marked with an asterisk in case of significant differences at P < 0.05.