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Figure 5 | BMC Plant Biology

Figure 5

From: Triparental origin of triploid onion, Allium × cornutum (Clementi ex Visiani, 1842), as evidenced by molecular, phylogenetic and cytogenetic analyses

Figure 5

Genomic in situ hybridisation (GISH) in Allium × cornutum ; a, b) and subsequent 5S rDNA mapping (c) to mitotic metaphase chromosomes of Allium × cornutum. (a, b) GISH with genomic DNA of A. pskemense (green), A. roylei (red) and A. cepa as blocking DNA (DAPI-blue); (a) Arrows and letters (C, R, and P) indicate the putative parental origins of the three genomes (A. cepa, A. roylei and A. pskemense, respectively); (b, c) The letters C, R, and P indicate chromosomes carrying 5S (green) and 35S (red) signals that belong to the three different parental genomes. C genome The 35S rDNA-carrying chromosome could not be identified; therefore it is not indicated. One nucleus (top left corner) visible in (b) was lost during reprobing and is not visible in (c).

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