Somatic embryogenesis, tetraploidy, and variant leaf morphology in transgenic diploid strawberry (Fragaria vesca subspecies vesca ‘Hawaii 4’)

Table 1 B/A ratios, nuclear DNA content values, of transgenic F. vesca ‘Hawaii 4’ plants

Name of plant line	B/A ratio	Inferred ploidy	DNA ratio with internal standard	Name of plant line	B/A ratio	Inferred ploidy	DNA ratio with internal standard
D09-66B	3.8	2x	0.24	E08-64	2.4	4x	0.48
E08-52	3.5	2x	0.24	B07-1	2.3	4x	0.46
D02-30	3.4	2x	0.23	F10-40	2.3	4x	0.46
E08-30	3.3	2x	0.25	E10-56	2.3	4x	0.46
E10-44	3.1	2x	0.23	E11-16	2.3	4x	0.48
B10-6	3.1	2x	0.23	E08-4	2.3	4x	0.48
E08-51	3.1	2x	0.24	E08-16	2.3	4x	0.46
G10-6	3.0	2x	0.23	F06-7	2.2	4x	0.45
F06-13	2.9	2x	0.24	F06-60	2.2	4x	0.48
D02-7	2.9	2x	0.23	D09-28	2.2	4x	0.48
C12-5	2.9	2x	0.23	D09-53	2.2	4x	0.48
F06-174	2.8	2x	0.23	D09-87	2.2	4x	0.48
C10-67	2.8	2x	0.23	E10-53	2.1	4x	0.47
B10-3	2.8	2x	0.25	G10-2	2.1	4x	0.46
E08-1	2.7	2x	0.23	D02-35	2.1	4x	0.48
E08-37	2.7	2x	0.23	C12-9	2.1	4x	0.47
F10-113	2.6	2x	0.23	F10-10	2.0	4x	0.46
D09-14	2.6	2x	0.24	E10-1	2.0	4x	0.45
B07-56	2.5	2x	0.23	G10-131	2.0	4x	0.46
F06-121	2.5	2x	0.23	C10-241	2.0	4x	0.46
Mean	2.95		0.23	D02-25	2.0	4x	0.47
				B10-20	2.0	4x	0.47
				C12-2	2.0	4x	0.47
				B07-12	1.9	4x	0.48
				C10-56	1.9	4x	0.46
				A05-7	1.8	4x	0.47
				F10-42	1.8	4x	0.45
				Mean	2.11		0.47

The internal standard used in DNA content measurements was Ilex crenata ‘Fastigiata’.

ISSN: 1471-2229