Figure 4

HR features are manifested early in the p5cdh mutant. Comparison of responses of wild type (wt) and p5cdh plants to infection by Pst-AvrRpm1. The abundance of ProDH and P5CDH transcripts (A) and proteins (B) was determined as described in the legend of Figure 1. C: ROS levels analyzed by H₂DCF-DA staining. D: Nuclei from non-viable cells detected with SYTOX Green dye. In C and D probe fluorescence was quantified with ImageJ software (bottom). Values represent mean ± SE of samples (3 infection experiments, 3 plants per genotype each; 32 images per genotype in C; and 18 in D). E: Pst-AvrRpm1 content in planta at the indicated times post-inoculation. Data represent means ± SD of one representative from 3 experiments. Control: uninoculated samples; I, II, III: samples corresponding to phases I, II, III of HR. Bars = 400 μm. *: significant differences between samples from wild type and mutant plants (p < 0.05 by t test).