Figure 2
From: Context of action of Proline Dehydrogenase (ProDH) in the Hypersensitive Response of Arabidopsis
In vivo14C-Pro consumption at phases I and II of HR. A: The 14C-Pro molecule used in the assay conserved all labeled carbons (*) after its conversion into 14C-P5C, and vice versa. B: Scheme of the experimental design applied to evaluate in vivo14C-Pro consumption in healthy and Pst-AvrRpm1-treated leaves (see Methods for details). C: PhosphorImager scan of a TLC plate including samples described in B. One representative plate from three independent biological experiments is shown. * indicates the origin of TLC plate. Pro, P5C and Glu positions, determined by ninhydrin staining, are indicated on the right. D: Densitometric analysis of the 14C-Pro content in the TLC plate using ImageJ software. Data are means ± SD of three independent experiments.