Q-PCR validation, results show gene expression estimated by Q-PCR and RNA-seq. Graphs show the log2 fold change in expression relative to morning, for midday and dusk samples. The Results are shown for QPCR estimates from the same samples in red and RNA-seq derived fold change shown in green. Also QPCR results shown from samples collect in the following year are shown in blue, where samples were only collected from mature leaves. The data is split between apical and mature leaves (left and right) and the seven genes tested.