Figure 3

Construction of GmPIP1;6 overexpression transgenic soybean. (A) Schematic illustration of T-DNA sequence of GmPIP1;6 overexpression vector. (B) Reverse transcript PCR analysis of GmPIP1;6 overexpression transgenic lines. 1 to 11 represented 11 independent T₁ generation GmPIP1;6 overexpression lines. WT, wild type. (C) qRT-PCR analysis of two representative GmPIP1;6 overexpression transgenic lines. RNA was extracted from the leaves of fourteen-day-old seedlings. All data are means of four biological replicates with error bars indicating SD. Expression of GmACTIN was used as the internal control.