Figure 2

Expression pattern of GmPIP1;6 under normal and NaCl treatments. (A) Relative expression levels of GmPIP1;6 in unifoliate leaf (UL), trifoliolate leaf (TL), stem (S), flower (F), pod (P) and root (R). Soybean seedlings were grown in nutrient solution. Total RNA was extracted from different tissues for qRT-PCR. (B) Ten-day-old soybean seedlings were treated with or without 100 mM NaCl in nutrient solution. RNA was extracted from the leaves of these seedlings at 6 hours, 12 hours, 1 day, 3 days, 5 days after treatment. All data are means of four biological replicates with error bars indicating SD. Expression level of treated plants was relative to control plants at each time point.