Table 1 Adaptor and primer sequences used
Primer name | 5′–3′ sequence | Usage |
|---|---|---|
Oligo d (T)18 | TTTTTTTTTTTTTTTTTT | Reverse transcription |
DPF | TGGCGGATGAAGATGCCNGARAC | Degenerated PCR |
DPR | ACCGGGCGGGGAADATYTCNGC | Degenerated PCR |
AAP | GGCCACGCGTCGACTAGTACGGGIIGGGIIGGGIIG | 5′ -RACE |
AUAP | GGCCACGCGTCGACTAGTAC | 5′ -RACE |
GSP5′-1 | TCCGAGCAAGTGAAGG | 5′ -RACE |
GSP5′-2 | AACCCAAATGAGTTGCTCTTGTCC | 5′ -RACE |
GSP5′-3 | GCCATATCTTGTGCAGCTTGT | 5′ -RACE |
dT-B26 | GACTCGAGTCAACATCGATTTTTTTTTTTTTTTTT | 3′ -RACE |
B26 | GACTCGAGTCAACATCGA | 3′ -RACE |
GSP3′-1 | GTGCTACCGGCGAGGTTTAT | 3′ -RACE |
GSP3′-2 | GTCCCTGGTTACTGGTTCACTG | 3′ -RACE |
Full-F | ATCTCCTTTTCTTCAATCACTCTCT | ORF amplifications |
Full-R | TCTAGGGTTTTATTCTTCATACCCT | ORF amplifications |
CmPT1-EcoR-F | CCGGAATTCCGGGTTTCGAAGGAGGAGTGTATCCATG | Functional complementation |
CmPT1-Not-R | ATTTGCGGCCGCAACAGTATTCAGTCAATCAAGCCGG | Functional complementation |
qGSP-F | CTCGCTCGTTGTTCTTGGTATC | qRT-PCR |
qGSP-R | AACAGTATTCAGTCAATCAAGC | qRT-PCR |
EF1A-F | TTTTGGTATCTGGTCCTGGAG | qRT-PCR |
EF1A-R | CCATTCAAGCGACAGACTCA | qRT-PCR |
35S-F | AGATACAGTCTCAGAAGACCAAAGG | Transgenic detection |
35S-R | TTGATATTCTTGGAGTAGACGAGAG | Transgenic detection |