Figure 4

Complementation assay of CmPT1 in yeast strain MB192. (a) Test for acid phosphatase activity. Yp112-CmPT1 carries the CmPT1 transgene, Yp112 is an empty vector, WT is wild type MB192. (b) Growth of MB192, Yp112-CmPT1, Yp112, and WT cells after 40 h growth in the presence of 60 mM Pi. (c) The effect of varying the pH of the medium on the growth of MB192, Yp112-CmPT1, Yp112 and WT. (d)³²P transport in Yp112-CmPT1 as a function of Pi concentration. A non-linear regression of Pi uptake by Yp112-CmPT1 at pH6 was used to estimate the K _m of Pi uptake. OD(600): optical density measured at 600 nm.