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Figure 1 | BMC Plant Biology

Figure 1

From: Mutation of the RDR1 gene caused genome-wide changes in gene expression, regional variation in small RNA clusters and localized alteration in DNA methylation in rice

Figure 1

OsRDR1 gene expression is abolished in the Tos17 insertion mutant Osrdr1 . (a) Structure of the OsRDR1 locus with the Tos17 insertion into the 2nd exon (vertical arrows). Heterozygous/homozygous individuals were selected based on PCR which primers were indicated by the purple arrows while primers for OsRDR1 expression analysis were indicated by black arrows. (b) Germinated seedling, paddy-field-grown plant and kernel phenotypes of wide-type (WT) and Osrdr1. (c) Relative to WT, OsRDR1 expression was silenced in shoots of Osrdr1, as evidenced by both qRT-PCR (top) and semi-quantitative RT-PCR (bottom) amplifications with gene-specific primers downstream of the Tos17 insertion (horizontal arrows). Genomic DNAs were used as positive controls.

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