Figure 3
From: Multiphoton imaging to identify grana, stroma thylakoid, and starch inside an intact leaf
Multiphoton laser scanning microscopic images of chloroplasts inside an intact leaf. The backward 2PF and forward SHG signals are presented as red and green colors, respectively. We choose three different regions to illustrate the distribution and meaning of colors. For each region, a line profile corresponds to the white dashed line is shown to the right. (arrow: grana; arrowhead: starch; dotted arrow: stroma thylakoid) (a) The chloroplast is filled with 2PF (red) overlapped with discrete SHG (green) spots. In the combined image, the red part corresponds to stroma thylakoid, and the yellow (=green + red) part corresponds to grana. (b) The distribution of 2PF is complementary to SHG. In the combined image, the red part still corresponds to stroma thylakoid, and the green part shows the location of starch granules. (c) In the combined image, yellow, green, and red correspond to grana, starch, and stroma thylakoid, respectively.