From: Tomato SlMKK2 and SlMKK4 contribute to disease resistance against Botrytis cinerea
Primers | Sequences (5′-3′) | Size (bp) |
---|---|---|
Cloning of cDNA | ||
SlMKK1-F | ATGAAGAAAGGATCTTTTGCAC | 1074 |
SlMKK1-R | TTATAGCTCAGTAAGTGTTGCC | |
SlMKK2-F | ATGCGACCAGCCGCCAACTCCA | 1080 |
SlMKK2-R | TCAAGAAGAGGAGGAAAAATGA | |
SlMKK3-F | ATGAAGACGGCGAAGCCATTGA | 1065 |
SlMKK3-R | TTATCTTGGAAAATTTACTGGG | |
SlMKK4-F | ATGGCCTTAGTTCGTGATCGCC | 1008 |
SlMKK4-R | TTAGGTGGATTTCAAATCGATA | |
SlMKK5-F | ATGGCTGGACTGGAGGAATTG | 1548 |
SlMKK5-R | CTATTGAGTAATGAAAAGTTC | |
VIGS constructs | ||
SlMKK1-VIGS-F | TGC TCTAGAGCAAAACCCCATTTGCCTGA | 360 |
SlMKK1-VIGS-R | CCG GAGCTCCTGATGGCTGTATAACTGAA | |
SlMKK2-VIGS-F | TGC TCTAGACAATCCAATACCATTATTCA | 360 |
SlMKK2-VIGS-R | TCCCCCGGGAAGACGGACAGAATCCTCGT | |
SlMKK3-VIGS-F | TGC TCTAGAGGGTTATTTGGGCGGTCCGT | 360 |
SlMKK3-VIGS-R | CCG GAGCTCAATGTTCCAACCCATTTATG | |
SlMKK4-VIGS-F | TGCTCTAGA ACTTGAAAAGCTTAAGGTTC | 400 |
SlMKK4-VIGS-R | CCGCTCGAG ACGATTCACTAAAAGGTTCG | |
SlMKK5-VIGS-F | TGC TCTAGAATGGCTGGACTGGAGGAATT | 360 |
SlMKK5-VIGS-R | CCG GAGCTCCTGCCTTTTCTCCTTCTCAA | |
Transient expression | ||
SlMKK2DD-GFP-F | GCTGTACAAGGGATCCATGCGACCTCTTCAACCACC | 1080 |
SlMKK2DD-GFP-R | TAATTAACTCTCTAGATTAAGAAGAAAAATGAGGAG | |
SlMKK4DD-GFP-2F | TGCTCTAGAATGGCCTTAGTTCGTGATCGCCG | 1008 |
SlMKK4DD-GFP-2R | TCCCCCGGGTTAGGTGGATTTCAAATCGATAC | |
qRT-PCR | ||
SlMKK1-RT-F | GGCCAATACCTTTGTCGGCACATA | 148 |
SlMKK1-RT-R | TCCCTCGGGTGGTTTATATGGGAA | |
SlMKK2-RT-F | AAGGTTCTACATCGTCCCACTGGA | 100 |
SlMKK2-RT-R | TCTCGATCTCACGGCACATCTGAA | |
SlMKK3-RT-F | AATGCTAGCCAGCTCTATGGGTCA | 181 |
SlMKK3-RT-R | AGCTTGCTGGTCCTCTGACTGTAT | |
SlMKK4-RT-F | CGCTAAGCAAGTGCTTGGTGGATT | 158 |
SlMKK4-RT-R | TGCAAGGATCCAAAGTCCTTCCCA | |
SlMKK5-RT-F | CCAGAACCTATCCTTTCCTCAAT | 103 |
SlMKK5-RT-R | GATTTGCTGGCTTTATGTCTCTG | |
SlActin-RT-F | CCAGGTATTGCTGATAGAATGAG | 113 |
SlActin-RT-R | GAGCCTCCAATCCAGACAC | |
SlPI I-RT-R | GTTGTACAAATGCCTGTGGTGAC | 135 |
SlPI I-RT-R | GGTAAGAGTACATGAAGAGATGC | |
SlPI II-RT-F | CATCTTCTGGATTGCCCA | 106 |
SlPI II-RT-R | ACACACAACTTGATGCCCAC | |
SlLapA-RT-F | GGGACTAATGATGTTTGGAA | 109 |
SlLapA-RT-R | GTGGCAATTTTATTTAGGCA | |
SlPR1b-RT-F | TTTCCCTTTTGATGTTGCT | 96 |
SlPR1b-RT-R | TGGAAACAAGAAGATGCAGT | |
SlPR-P2-RT-F | CGATCTAAATTGATTTCATAGTACG | 116 |
SlPR-P2-RT-R | TCGTGAAGGATATACAAAATACA | |
BcActin-RT-F | CGTCACTACCTTCAACTCCATC | 107 |
BcActin-RT-R | CGGAGATACCTGGGTACATAGT | |
NbActin-RT-F | ACCAGATTAATGAGCCCAAGAG | 97 |
NbActin-RT-R | CCAACAGGGACAGTACCAATAC | |
NtPR1-RT-F | CCGTTGAGATGTGGGTCAAT | 100 |
NtPR1-RT-R | CGCCAAACCACCTGAGTATAG | |
NtPR2-RT-F | CAACCCGCCCAAAGATAGTA | 98 |
NtPR2-RT-R | TGGCTAAGAGTGGAAGGTTATG | |
NtPR4-RT-F | GGATGATGTTGACAGCAGAGA | 116 |
NtPR4-RT-R | GTAGGACACGAGGTAGGTATCA | |
NtPR5-RT-F | GCTCGATTACGTCTTGTCTCTC | 104 |
NtPR5-RT-R | CTCTAGCATGGTGGATTGACTT | Â |