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Table 1 Primers used in this study for different purposes

From: Tomato SlMKK2 and SlMKK4 contribute to disease resistance against Botrytis cinerea

Primers

Sequences (5′-3′)

Size (bp)

Cloning of cDNA

SlMKK1-F

ATGAAGAAAGGATCTTTTGCAC

1074

SlMKK1-R

TTATAGCTCAGTAAGTGTTGCC

SlMKK2-F

ATGCGACCAGCCGCCAACTCCA

1080

SlMKK2-R

TCAAGAAGAGGAGGAAAAATGA

SlMKK3-F

ATGAAGACGGCGAAGCCATTGA

1065

SlMKK3-R

TTATCTTGGAAAATTTACTGGG

SlMKK4-F

ATGGCCTTAGTTCGTGATCGCC

1008

SlMKK4-R

TTAGGTGGATTTCAAATCGATA

SlMKK5-F

ATGGCTGGACTGGAGGAATTG

1548

SlMKK5-R

CTATTGAGTAATGAAAAGTTC

VIGS constructs

SlMKK1-VIGS-F

TGC TCTAGAGCAAAACCCCATTTGCCTGA

360

SlMKK1-VIGS-R

CCG GAGCTCCTGATGGCTGTATAACTGAA

SlMKK2-VIGS-F

TGC TCTAGACAATCCAATACCATTATTCA

360

SlMKK2-VIGS-R

TCCCCCGGGAAGACGGACAGAATCCTCGT

SlMKK3-VIGS-F

TGC TCTAGAGGGTTATTTGGGCGGTCCGT

360

SlMKK3-VIGS-R

CCG GAGCTCAATGTTCCAACCCATTTATG

SlMKK4-VIGS-F

TGCTCTAGA ACTTGAAAAGCTTAAGGTTC

400

SlMKK4-VIGS-R

CCGCTCGAG ACGATTCACTAAAAGGTTCG

SlMKK5-VIGS-F

TGC TCTAGAATGGCTGGACTGGAGGAATT

360

SlMKK5-VIGS-R

CCG GAGCTCCTGCCTTTTCTCCTTCTCAA

Transient expression

SlMKK2DD-GFP-F

GCTGTACAAGGGATCCATGCGACCTCTTCAACCACC

1080

SlMKK2DD-GFP-R

TAATTAACTCTCTAGATTAAGAAGAAAAATGAGGAG

SlMKK4DD-GFP-2F

TGCTCTAGAATGGCCTTAGTTCGTGATCGCCG

1008

SlMKK4DD-GFP-2R

TCCCCCGGGTTAGGTGGATTTCAAATCGATAC

qRT-PCR

SlMKK1-RT-F

GGCCAATACCTTTGTCGGCACATA

148

SlMKK1-RT-R

TCCCTCGGGTGGTTTATATGGGAA

SlMKK2-RT-F

AAGGTTCTACATCGTCCCACTGGA

100

SlMKK2-RT-R

TCTCGATCTCACGGCACATCTGAA

SlMKK3-RT-F

AATGCTAGCCAGCTCTATGGGTCA

181

SlMKK3-RT-R

AGCTTGCTGGTCCTCTGACTGTAT

SlMKK4-RT-F

CGCTAAGCAAGTGCTTGGTGGATT

158

SlMKK4-RT-R

TGCAAGGATCCAAAGTCCTTCCCA

SlMKK5-RT-F

CCAGAACCTATCCTTTCCTCAAT

103

SlMKK5-RT-R

GATTTGCTGGCTTTATGTCTCTG

SlActin-RT-F

CCAGGTATTGCTGATAGAATGAG

113

SlActin-RT-R

GAGCCTCCAATCCAGACAC

SlPI I-RT-R

GTTGTACAAATGCCTGTGGTGAC

135

SlPI I-RT-R

GGTAAGAGTACATGAAGAGATGC

SlPI II-RT-F

CATCTTCTGGATTGCCCA

106

SlPI II-RT-R

ACACACAACTTGATGCCCAC

SlLapA-RT-F

GGGACTAATGATGTTTGGAA

109

SlLapA-RT-R

GTGGCAATTTTATTTAGGCA

SlPR1b-RT-F

TTTCCCTTTTGATGTTGCT

96

SlPR1b-RT-R

TGGAAACAAGAAGATGCAGT

SlPR-P2-RT-F

CGATCTAAATTGATTTCATAGTACG

116

SlPR-P2-RT-R

TCGTGAAGGATATACAAAATACA

BcActin-RT-F

CGTCACTACCTTCAACTCCATC

107

BcActin-RT-R

CGGAGATACCTGGGTACATAGT

NbActin-RT-F

ACCAGATTAATGAGCCCAAGAG

97

NbActin-RT-R

CCAACAGGGACAGTACCAATAC

NtPR1-RT-F

CCGTTGAGATGTGGGTCAAT

100

NtPR1-RT-R

CGCCAAACCACCTGAGTATAG

NtPR2-RT-F

CAACCCGCCCAAAGATAGTA

98

NtPR2-RT-R

TGGCTAAGAGTGGAAGGTTATG

NtPR4-RT-F

GGATGATGTTGACAGCAGAGA

116

NtPR4-RT-R

GTAGGACACGAGGTAGGTATCA

NtPR5-RT-F

GCTCGATTACGTCTTGTCTCTC

104

NtPR5-RT-R

CTCTAGCATGGTGGATTGACTT

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