Skip to main content
Figure 7 | BMC Plant Biology

Figure 7

From: Tomato SlMKK2 and SlMKK4 contribute to disease resistance against Botrytis cinerea

Figure 7

Transient expression of constitutively active phosphomimicking forms SlMKK2DDand SlMKK4DDin Nicotiana benthamiana resulted in hypersensitive response and accumulation of ROS. A. Expression of SlMKK2DD and SlMKK4DD. Gene expression was analyzed by qRT-PCR and relative expression levels were calculated by comparing with the corresponding values at 0 hr (as a control) after infiltration. B. Proteins of SlMKK2DD and SlMKK4DD. Leaf samples were harvested 48 hr after infiltration and total soluble protein extracts were prepared. Proteins were separated by SDS–PAGE and analyzed by immunoblotting using a GFP-specific antibody. Total proteins showing equal loading were examined by Coomassie staining. C. HR-like cell death. Photo was taken 48 hr after infiltration. D. Accumulation of H2O2. Detection of H2O2 was performed by DAB staining at 48 hr after infiltration. Data presented in (A) are the means ± SD from three independent experiments and different letters above the columns indicate significant differences at p < 0.05 level.

Back to article page