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Figure 9 | BMC Plant Biology

Figure 9

From: An Arabidopsis mutant impaired in intracellular calcium elevation is sensitive to biotic and abiotic stress

Figure 9

Induction of camalexin, indolic glucosinolates (iGLS), aliphatic glucosinolates (aGLS) and the genes involved in their biosynthesis in WT and cycam1 after A. brassicae infection. The leaves of 12-day old WT, cycam1-1 and cycam1-2 seedlings grown on MS under LD conditions were transferred to PNM media as described in Methods, inoculated with a fungal spore suspension containing 104 to 105 cfu ml−1 and incubated under LD condition for additional 3 days before determination of camalexin (relative quantification) (A), iGLS (B) and aGLS (D) levels. iGLS are I3M (indolyl-3-methyl-GLS), 4MOI3M (4-methoxyindolyl-3-methyl-GLS) and 1MOI3M (1-methoxyindolyl-3-methyl-GLS). aGLS are 5MSOP (5-methylsulfinylpentyl-GLS) and 7MSOH (7-methylsulfinyl-heptyl-GLS). mRNA levels of camalexin and iGLS (C) and aGLS (E) biosynthesis genes in the leaves of WT, cycam1-1 and cycam1-2 2 dai with A. brassicae. Mock treatment was performed with sterile water. The abbreviations of the gene and annotation numbers are given. The mRNA levels for each cDNA were normalized with respect to the plant GAPDHC message levels. The mRNA level for mock-treated WT seedlings was set as 1.0 and the other values refered to it. The values are means ± SEs of four independent RT-PCR experiments with three replications in each experiment. For (A), (B) and (C), all values (+ A. brassicae) are significantly different from the values (- A. brassicae) with P < 0.01. For (D) and (E), asterisks indicate significant differences, as determined by Student’s t-test (* P > 0.1; ** P < 0.01).

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