Figure 7

SDS-PAGE analysis of the recombinant TcPR-4b protein. M. Molecular weight marker (Thermo Scientific). Lane 1: pET28a without insert and without induction. Lane 2: pET28a without insert and 4 h after induction with 1 M of IPTG at 37°C. Lane 3: pET28a-TcPR-4b 1 h after induction with 1 M of IPTG at 37°C. Lane 4: pET28a-TcPR-4b 2 h after induction with 1 M of IPTG at 37°C. Lane 5: pET28a-TcPR-4b 3 h after induction with 1 M of IPTG at 37°C. Lane 6: pET28a-TcPR-4b 4 h after induction with 1 M of IPTG at 37°C. Lane 7: pET28a-TcPR-4b 1 h after induction with 0.4 M of IPTG at 37°C. Lane 8: pET28a-TcPR-4b 2 h after induction with 0.4 M of IPTG at 37°C. Lane 9: pET28a-TcPR-4b 3 h after induction with 0.4 M of IPTG at 37°C. Lane 10: pET28a-TcPR-4b 4 h after induction with 0.4 M of IPTG at 37°C. Lane 11: pET28a-TcPR-4b after induction overnight with 1 M of IPTG at 18°C. Lane 12: pET28a-TcPR-4b after induction overnight with 0.4 M of IPTG at 18°C. Lane 13: purified insoluble fraction of the recombinant TcPR-4b (15.43 kDa). The arrow indicates the recombinant TcPR-4b protein.