Figure 3

Calcofluor white stained wild type and Aa ttg1 mutant seeds. Fluorescence microscope (A,B,E,F,I,J,M,N) and CLSM (fluorescence channel: C,G,K,O, transmission channel: D,H,L,P) pictures of the surface of A. alpina seeds stained with calcofluor white. A to D) wild type Paj. I to L) pep1-1 mutant. Note, that in wild type and pep1-1 mutants the columella is seen as large domes. E to H) Aattg1-1 mutant induced in the wild type Paj background. M to P) The Aattg1-2 mutant induced in the pep1-1 background. In both mutants only the rim of the epidermal cells is left and columellas are absent. Scale bar: A,E,I,M = 300 μm, B,F,G,N = 100 μm, C,D,G,H,K,L,O,P = 50 μm.