Figure 2

TTG1 is needed for the accumulation of anthocyanidins in A. alpina . Seeds (A-C) and seedlings (D-F) of Aattg1-1 and Aattg1-2 are devoid of cyanidin - a late component of the (pro-) anthocyanidin biosynthesis pathway - but contain kaempferol – an early component of this pathway. The genotype for each row in A-C and D-F is given on the right. A) stereo-microscopy of seeds. Aattg1 mutants have a yellowish seed colour as compared to the respective backgrounds indicating a lack of proanthocyanidin; B) extracted and hydrolyzed insoluble components of the seeds‘ (pro-) anthocyanidin pathway, presence (backgrounds) or absence (Aattg1 mutants) of pink colour relates to the presence or absence of (pro-) anthocyanidin in seeds; C), F) HPLC-MS analysis of extracted soluble, hydrolyzed components of the anthocyanidin biosynthesis pathway. Shown are extracted ion chromatograms for m/z = 287.055 +/- 0.005. Note that cyanidin and kaempferol have the same m/z value. Different scales were chosen to highlight the absence of cyanidin in F). Full chromatograms are provided in Additional file 3: Figure S2. D) photography of 5 day-old seedlings grown on MS medium with 1% sucrose at constant light. E) zoom in on the petiole- and SAM-region of the seedlings shown on the left in D). Aattg1 mutants do not accumulate anthocyanidins in the seedling‘s hypocotyl. All pictures within one subfigure were taken at the same light settings. Bar in A: 1 mm; bar in C: 2 mm; 1: cyanidin (late biosynthesis compound); 2: kaempferol (early biosynthesis compound).