Figure 3

Sequence analysis of single base deletion in pseudogenes TaHKT2;1 7AL-2/ TaHKT2;1 7AL-3 from different Triticum species . (A) Gene structure and partial DNA sequence with predicted protein showing pre-mature stop codon indicated by an asterisk. (B) Nullisomic- tetrasomic analysis of gene specific primer pair 5′A2/3 F and 5′A2/3 R for amplifying and sequencing the region coding for premature stop codon. (C) Sequence chromatogram comparing the functional TaHKT2;1 7AL-1 with TaHKT2;1 7AL-2/TaHKT2;1 7AL-3 where the single base deletion is highlighted by an arrow. (D) Sequences of the functional gene TaHKT2;1 7AL-1, the conserved single base deletion at +191 bp in TaHKT2;1 7AL-2/ TaHKT2;1 7AL-3 in Chinese Spring and in selected diploid, tetraploid and hexaploid Triticum species. Single base deletion is shown by dash whereas SNPs between accessions are indicated in blue.