Fluorescence parameters | Fluorescence parameters description |
---|---|
Extracted parameters | |
Ft | Fluorescence intensity at time t after onset of actinic illumination |
F50 μs | Minimum reliable recorded fluorescence at 50 μs with the Handy PEA |
Fk (F300 μs) | Fluorescence intensity at 300 μs |
FP | Maximum recorded (=maximum possible) fluorescence at P-step |
Area | Total complementary area between fluorescence induction curve and F = Fm |
Derived parameters | |
Fo ≌ F50 μs | Minimum fluorescence, when all PSII RCs are open |
Fm = FP | Maximum fluorescence, when all PSII RCs are closed |
Vj = (Fj - Fo)/(Fm - Fo) | Relative variable fluorescence at the J-step (2 ms) |
Vi = (Fi - Fo)/(Fm - Fo) | Relative variable fluorescence at the I-step (30 ms) |
WK = (Fk - Fo)/(Fj - Fo) | Representing the damage to oxygen evolving complex (OEC) |
Mo = 4 (Fk - Fo)/(Fm - Fo) | Approximated initial increment (in ms-1) of the relative variable fluorescence |
Fv/Fm = 1- (Fo/Fm) | Maximum quantum yield of primary photochemistry at t = 0 |
φEo = ETo/ABS = (Fm-Fj)/Fm | Quantum yield for electron transport at t = 0 |
RCQA = φPo × (ABS/CSm) × (Vj/Mo) | Amount of active PSII RCs (QA-reducing PSII reaction centers) per CS at t = m |