Figure 2

SMV resistance and GmAKT2 expression in three soybean genotypes. (A) Symptoms of three soybean genotypes at 14 and 28 DAI. Rsmv1, SMV highly-resistant; Williams 82, susceptible; Ssmv1, highly-susceptible. Ten-day-old soybean plants in low-K soil with unrolled unifoliate leaves were mechanically inoculated with SMV G7 or buffer (Mock). (B) Amount of SMV RNA detected by qRT-PCR. Leaves of three soybean genotypes were sampled at 14 and 28 DAI to extract total RNA for qRT-PCR analysis. Transcript levels were calculated using the formula 2^-ΔCt for the expression levels relative to GmACTIN. Data represent means of three biological replicates with error bars indicating SD. (C) Relative GmAKT2 expression in three soybean genotypes at 28 DAI. Leaves of three soybean genotypes were sampled at 28 DAI to extract total RNA for qRT-PCR analysis. Transcript levels were calculated using the formula 2^-ΔΔCt for the expression levels relative to GmACTIN. Data represent means of three biological replicates with error bars indicating SD.