Contents of proline and sugars in 35S: BdCBF1 transgenic Brachypodium plants. Five measurements were averaged and statistically treated using the Student t-test (*P < 0.01). Bars indicate standard error of the mean. A. Proline contents. Two-week-old plants grown on MS-agar plates were exposed to either 300 mM NaCl for 24 h or 4°C for 24 h. Whole plants were used for the extraction of proline. Contents of free proline were measured by HPLC, as described previously . The numbers in parentheses indicate fold changes relative to the value in the mock-treated Bd21-3 plants. Bars indicate standard error of the mean. B. Sugar contents. The plant materials described in (A) were used for the extraction of soluble sugars. Contents of individual sugars were determined by HPLC, as described previously . Mo, mock.