Effects of abiotic stresses on the expression of BdCBF genes. Two-week-old Brachypodium plants grown on MS-agar plates were exposed to cold (4°C) for the indicated time durations. The plants were also floated in MS liquid media containing either 400 mM mannitol, 300 mM NaCl, or 100 μM ABA for the indicated durations. Whole plants were used for the extraction of total RNA samples. Transcript levels were determined by qRT-PCR. Biological triplicates were averaged and statistically treated using the Student t-test (*P < 0.01). Bars indicate standard error of the mean. A. Effects of abiotic stresses on BdCBF1 gene expression. B. Effects of abiotic stresses on BdCBF2 gene expression. C. Effects of abiotic stresses on BdCBF3 gene expression.