Amino acid sequence comparison of BdCBFs with monocot CBFs. A. Multiple sequence alignment of the AP2 domains of CBF proteins. Amino acid sequences of the AP2 domains were aligned using ClustalX (http://www.clustal.org/clustal2/). Black boxes indicate identical residues, and gray boxes indicate biochemically conserved residues. The AP2/EREBP and DSAWR signature sequences are marked by brackets. Abbreviations for plant species are as follows; Bd, Brachypodium distachyon; Hv, Hordeum vulgare; Os, Oryza sativa; Sb, Sorghum bicolor; Sc, Secale cereale; Ta, Triticum aestivum; Zm, Zea mays. B. Phylogenetic analysis of monocot CBF proteins. The phylogenetic tree was constructed using the MEGA5 software . Bootstrap values (>50%) based on 1000 replicates are shown.