SbCO promotes flowering by inducing SbEHD1 and FT -like genes in LD (14 h light/10 h dark). A. Flowering time phenotype of RIL112 and RIL105. (Days to flowering for RIL112 and RIL105 are 113 and 75.) B-F. Relative expression levels of flowering time genes in RIL105 (black solid line) and RIL112 (red dashed line). Gray shading denotes the dark/night portion of each 24 h cycle. The first 24 h covers one light–dark cycle, followed by 24 h of continuous light and temperature (LL). B. SbCO. C. SbEHD1. D. SbCN8. E. SbCN12. F. SbCN15. G. Average fold differences of the first 24 h (light–dark cycle) between the mRNA levels of each gene in RIL105 and RIL112 is plotted. Positive values represent higher expression detected in RIL105. Each expression data point corresponds to three technical replicates within three biological replicates.