The NaTrxh C-terminus contributes to the NaTrxh: S-RNase interaction. Pull-down experiments were performed using columns with the different NaTrxh versions [(A) NaT: NaTrxh; (B) NaTΔα: NaTrxhΔNα; (C) NaTΔαβ: NaTrxhΔNαβ; (D) NaTΔCOO: NaTrxhΔCOO]. Dotted lines indicate deleted regions. Stylar proteins from S105S105N. alata were passed through each column, and then, each recovered fraction was analysed by western blot immune-staining with anti-S105-RNase. UB: unbound fraction; W1, W5, and W10: first, fifth, and tenth washes, respectively, with binding buffer; Tw: binding buffer plus 0.1% Tween-20; NaCl 0.1 and 0.2: washes with 50 mMTris, pH 7.9 + 100 mM or 200 mM NaCl, respectively; B1 and B2: bound fractions eluted with one and two bed volumes of elution buffer.