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Figure 5 | BMC Plant Biology

Figure 5

From: A novel motif in the NaTrxh N-terminus promotes its secretion, whereas the C-terminus participates in its interaction with S-RNase in vitro

Figure 5

Nβ domain contributes to NaTrxh stability. N- and C-termini are not involved in its reductase activity. (A) SDS-PAGE analysis of different NaTrxh versions fused to GST expressed in E. coli cells. Upper panels: Coomassie blue stained gel; lower panels: western-blot immuno-stained with anti-NaTrxh antibody. S: soluble fraction; I: insoluble fraction; GST: gluthathione S-transferase; NaT: full-length NaTrxh; NaTΔα: NaTrxhΔNα mutant; NaTΔβ: NaTrxhΔNβ; NaTΔαβ: NaTrxhΔNαβ; NaTΔCOO: NaTrxhΔCOO. Arrows indicate the signal corresponding to the different NaTrxh:GST versions. (B) Thioredoxin activity assay using insulin as substrate and DTT as electron donor (Holmgren, 1979). Circles: NaTrxh; Diamonds: NaTrxhΔNα; Squares: NaTrxhΔNαβ; Triangles: NaTrxhΔCOO; Crosses: only DTT.

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BMC Plant Biology

ISSN: 1471-2229

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