Figure 6
AtUCP1 -overexpressing plants performed better under abiotic stress, most likely by decreasing ROS accumulation. (A) Two-week-old WT and P07 plantlets were plated on half-strength MS medium supplemented with 80 mM mannitol (hyperosmotic stress) or 10 mM H2O2 (oxidative stress), pH 7.5 and pH 4.5 for 22 days under a day/light regime of 16 h at 100 μE m−2 s−1 and 8 h in darkness. For the low-temperature treatment, the seeds were germinated and grown for 30 days on half-strength MS medium supplemented with 50 μM:10 μM linoleic acid:bovine serum albumin. Plant growth assays were performed using four biological replicates (n = 4) on plates containing 5 plants for each treatment. *p < 0.05 and **p < 0.1 compared with WT. Error bars, mean ± s.e.m. Scale bars, 200 μm. (B) Leaf discs (0.8 cm) or (C) root tips isolated from WT and the P07 transgenic line were incubated in half-strength MS medium supplemented with 100 mM NaCl, 100mM mannitol, or 10 mM H2O2 for 20 h. Control treatments were performed using unsupplemented half-strength MS medium. Tissues were stained with MitoSox red and visualized with fluorescent confocal microscopy.